Library Preparation#

Collecting Samples#

Illumina Library Preparation#

The lab uses this kit for prepare the library for sequencing.

Numbers of an Illumina Sequencing Run#

Illumina High Seq 4000 1. Cost per flow cell ~ S$2700 2.

HiSeq X

Table. Data Output for Common NGS Applications | Application | Estimated Data Output | |-------------------------------------------------|-----------------------| | Human whole-genome sequencing (at 30× coverage) | ~120 Gb | | Human exome sequencing (at 100× coverage) | ~8 Gb | | Microbial whole-genome sequencing | ~300 Mb | | 16S rRNA sequencing | ~60 Mb |